α-甲酰辅酶A消旋酶、核基质蛋白在前列腺癌早期诊断敏感性比较.docVIP

α-甲酰辅酶A消旋酶、核基质蛋白在前列腺癌早期诊断敏感性比较 　 作者：刘志明，李泽惠，李志鹏 【摘要】 目的 检测前列腺癌和非前列腺癌患者前列腺液中α-甲酰辅酶A消旋酶（AMACR）、核基质蛋白（NMP）的含量，分析比较各指标对早期诊断的特异性和敏感性。方法 64例前列腺癌标本（年龄在50岁以上）和24例对照组标本均来自泌尿外科住院患者和门诊患者。用前列腺按摩的方法获取前列腺癌患者前列腺液64例，非前列腺癌患者共24例作为对照组。标本放于-70 ℃冰箱保存，在同一时间内用双抗体夹心酶联免疫反应（ELISA）检测标本。结果 α-甲酰辅酶A消旋酶在64例前列腺癌患者的前列腺液里有很高敏感性，敏感性为96.9%（62/64），而且是非前列腺癌患者的20倍以上，具有显著统计学意义（P＜0.01），仅有2例α-甲酰辅酶A消旋酶浓度和对照组没有区别（P＞0.05）,64例前列腺癌患者的核基质蛋白是对照组的4倍，敏感性为92.2%。结论 AMACR具有很高的敏感性和特异性，比NMP具有更高的敏感性和特异性。 【关键词】 前列腺癌；诊断；α-甲酰辅酶A消旋酶；核基质蛋白 ［Abstract］ Objective To detect α-methylacyl-CoA racemase（AMACR），nuclear matrix protein（NMP）of prostate cancer and non-prostate cancer in prostatic fluid.To analyze and compare various indicators on early diagnosis specific and sensitivities.Methods 64 example of prostate cancer specimen（aged over 50 years old）and 24 example of control group specimen came from urology in-patient and the outpatients.With method of prostate massage to get the prostatic fluid of prostate cancer patients of 64 cases,non-prostate cancer patients were with a total of 24 patients as a control group,specimens.Placed in-70 ℃ refrigerator,at the same time using enzyme-linked immune response（ELISA）to test specimens.Results α-methylacyl coenzyme A racemase in 64 cases of prostate cancer in prostate fluid had a high sensitivity，and the sensitivity was 96.9%（62/64）,20 times higher than non-prostate cancer with significant significance（Plt;0.01）.Only two cases of α-methylacyl coenzyme A racemase levels in the control group had no difference（Pgt;0.05）.64 cases with prostate cancer of the prostatic nuclear matrix proteins level closed to 4 times higher than the control group,the sensitivity was 92%.Conclusion α-methylacyl-CoA racemase（AMACR）has a higher degree of sensitivity and specificity,than nuclear matrix protein（NMP）. ［Key words］ prostate cancer;diagnosis;α-methylacyl-CoA racemase;nuclear matrix protein 为检测前列腺癌和非前列腺癌患者前列腺液中α-甲酰辅酶A消旋酶（α-methylacyl-coenzyme A racemase,AMACR）、核基质蛋白（nuclear matrix protein,NMP）的含量，我们对6