Fas、FasL在特发性血小板减少性紫癜患者T细胞亚群表达探究.docVIP

Fas、FasL在特发性血小板减少性紫癜患者T细胞亚群表达探究 　 作者：洪丽，鲁田夫，吴立连，胡艳明 【摘要】 目的 探讨Fas、FasL在特发性血小板减少性紫癜(ITP)患者T细胞亚群及血小板中的表达状况及其在ITP发病机制中的作用。方法 收集ITP患者及健康人群外周抗凝静脉血，分离纯化得T细胞和血小板。以PE标记的antiCRTH2mAb和Cy5标记的antiCD4、CD8mAb及FITC标记的antiFas、FasL mAb做三色流式细胞术，分析ITP患者Th/Tc、Th1/Th2、Tc1/Tc2细胞Fas、FasL表达的变化;以FITC标记的antiFas、FasL mAb作单色流式细胞术，分析ITP患者血小板Fas、FasL表达的变化。结果 与健康人群相比，ITP患者外周血Th、Th1、Th2细胞百分率均明显下降(Plt;0.05)，Tc、Tc1、Tc2细胞百分率均无明显变化(Pgt;0.05);Th、Th1、Th2及Tc、Tc1、Tc2细胞Fas、FasL表达均明显升高(Plt;0.05);同时，ITP患者血小板Fas的表达明显升高(Plt;0.05)，而FasL的表达无明显变化(Pgt;0.05)。结论 ITP患者存在免疫功能失调，T细胞亚群及血小板Fas、FasL表达异常可能与ITP患者免疫病理机制有关。 【关键词】 特发性血小板减少性紫癜;Fas/FasL;T细胞亚群;血小板 　　ABSTRACT：Objective To investigated the expression of Fas, FasL on T cell subsets and thrombocyte in patients with idiopathic thrombocytopenic purpura (ITP) and its role in ITP pathogenesis. Methods Anticoagulated venous blood by heparin was collected from idiopathic thrombocytopenic purpura patients and healthy people and T cells and thrombocytes were separated by Human CD3+ T cell enrichment column. The expression of Fas, FasL on T cell subsets was detected by immunofluorescence staining and threecolor flow cytometry by Fas/CD4, FasL/CD4, Fas/CD8 and FasL/CD8 gating. The expression of Fas, FasL on thrombocytes was metried by immunofluorescence staining and monocolor flow cytometry by Fas and FasL gating. Results Compared with healthy people, the percentage of Th, Th1 and Th2 cells in peripheral blood of ITP patients was markedly decreased, and the expression of Fas, FasL on Th, Th1, Th2, Tc, Tc1 and Tc2 was significantly increased(Plt;0.05).Meanwhile, the expression of Fas on thrombocytes was also significantly elevated.Conclusion There is immunofunction disorder in ITP patients.,and the abnormal expression of Fas, FasL on T cell subsets and thrombocytes may involve in ITP immunopathogenesis. 　　KEY WORDS：Idiopathic thrombocytopenic purpura;Fas/FasL;T cell subsets;Thrombocyte 　　特发性血小板减少性紫癜(idiopathic thrombocytopenic purpura，ITP)是一种自身免疫性疾病，发病机制包括T、B细胞的异常活化和T细胞依