FHIT抑癌基因在乳腺癌组织中表达缺失.docVIP

FHIT抑癌基因在乳腺癌组织中表达缺失 　作者：金春亭 李海军 张林西 李玉珍 范婕 武欣 白美玲 【摘要】 目的：探讨FHIT基因蛋白在乳腺癌中的表达特点及其与临床病理参数的关系。方法：采用免疫组织化学SP法，检测62例乳腺癌组织中FHIT蛋白的表达情况。结果：乳腺癌组织中FHIT蛋白阳性表达率为54.84%，其表达缺失率为45.16%。FHIT蛋白在导管内癌局部浸润组的阴性表达率为30%，明显低于浸润性导管癌组（48.08%），但是没有统计学差异；临床Ⅰ～Ⅱ期阴性表达率为38.30%，明显低于Ⅲ～Ⅳ期阴性表达率（66.67%）（P=0.055）。FHIT蛋白在有淋巴结转移组表达缺失率为64.00%，显著高于无淋巴结转移组（32.43%）（P=0.014）。FHIT蛋白的表达缺失与患者年龄、癌的左右乳分布没有显著性差异。结论：乳腺癌组织中有FHIT蛋白的表达缺失，可能参与或促进乳腺癌的形成。 【关键词】 FHIT；乳腺癌；免疫组织化学 【ABSTRACT】 Objective: To investigate the expression characteristics of FHIT protein in breast carcinomas and its relationship with clinicopathological parameters. Methods: Expression of FHIT protein in breast carcinoma tissues of 62 cases was detected by SP immunohistochemistry. Results: The positive rate of FHIT protein expression in breast carcinoma tissues was 54.84%, and about 45.16% cases lost FHIT expression. Negative rate of FHIT expression in early invasive ductal carcinoma in situ group was obviously lower than that in invasive ductal carcinomas group (48.08%), but the difference wasnt significant. Also, loss of FHIT expression in stage Ⅲ～Ⅳ was higher than that in stage Ⅰ～Ⅱ(P=0.055). There was 64.00% breast carcinomas with lymph node metastasis lossing FHIT expression, the lossing rate was significantly higher than that in carcinomas without metastasis (P=0.014). FHIT expression had no relationship with patients age, distri bution of cancers in right or left breast. Conclusion: There was remarkable loss of FHIT protein expression in breast carcinomas. Loss of FHIT expression may be involved in the carcinogenesis of breast or progression of breast carcinomas. 【KEY WORDS】 FHIT; Breast carcinoma; Immunohistochemistry 脆性组氨酸三联体(Fragile histidine triad, FHIT)基因是位于染色体3p14.2上的一个候选抑癌基因，其改变主要见于上皮起源的人类恶性肿瘤。研究已发现FHIT基因在多种肿瘤[1～5]中均有改变且以缺失为主。为了明确FHIT蛋白表达缺失与乳腺癌患者的临床病理参数的相关性及其可能的预后，我们应用免疫组织化学方法对62例乳腺癌组织中FHIT蛋白的表达情况进行了检测。 1 材料与方法 1.1 标本来源 收集我院病理教研室2002.1～2004.4月乳腺癌术后送检标本62例，年龄25～73岁，平均年龄50.89岁。临床分期Ⅰ期16例，Ⅱ期31例，Ⅲ期10例，Ⅳ期5例。所有标本均经10%福尔马林液固定，石蜡包埋，4μｍ连