FHIT及C-erbB-2在乳腺癌进展过程中表达及意义.docVIP

FHIT及C-erbB-2在乳腺癌进展过程中表达及意义 　【摘要】 目的 探讨FHIT和C-erbB-2在乳腺癌进展过程中的表达和意义。方法 用免疫组化S-P法检测105例乳腺癌，50例乳腺良性肿瘤；40例正常乳腺对照组织（取自乳腺良性肿瘤以外2 cm的乳腺组织）组织中FHIT蛋白、C-erbB-2蛋白的表达。结果 FHIT蛋白在乳腺癌组织中的阳性率明显低于正常乳腺组织或乳腺良性肿瘤,比较差异有统计学意义（Plt;0.05）。C-erbB-2在乳腺正常组织、乳腺良性肿瘤及乳腺癌组织中的阳性率分别为0（0/40）,20%（10/50）,和76.2%（80/105）,比较差异有统计学意义（Plt;0.05）;在乳腺肿瘤组织中,随组织学病理分级的增加、临床分期的进展和腋窝淋巴结的转移,FHIT蛋白表达阳性率逐渐下降, C-erbB-2表达强阳性率则逐渐上升,比较差异有统计学意义（Plt;0.05）;FHIT蛋白、C-erbB-2蛋白表达相关（Plt;0.001）。结论 FHIT、C-erbB-2在乳腺癌的发生、发展过程中起着重要的作用,联合检测可为乳腺癌的早期诊断、进一步治疗及预后判断提供必要的理论依据。 【关键词】 乳腺肿瘤 FHIT C-erbB-2 免疫组织化学 The expression and significance of FHIT,C-erbB-2 in progression of breast carcinoma ［Abstract］ Objective To study the expression and significance of FHIT protein, C-erbB-2 protein in progression of breastcarcinoma.Methods Im-munohistochemical staining S-P method was used to detect the expression of FHIT protein, C-erbB-2protein in 105cases with breast carcinoma, 50 cases with breast benign and 40 cases normal mammary.Results The positive expression rate of FHIT protein in breast carcinoma was lower than that in normal breast （Plt;0.05）. the expressions rates of C-erbB-2 in the tissues of normal mammary, breast benign and breast carcinoma were 0（0/40）, 20%（10/50）and 76.2%（80/105） respectively,and there were statistical significance compared with each other （Plt;0.05）.With the increase of histological grade, clinic stage, lymphatic metasta-sis, the expression rate of FHIT was decreased, while the expression rate of Survivin was increased. Correlation was also found between FHIT and C-erbB-2 expressions.Conclusion FHIT and Survivin may play important roles in oncogenesis and progression of breast carcinoma. Detection of FHIT,Survivin protein may have potential effects on early diagnosis, treatment and prognosis of breast carcinoma. ［Key words］ breast neoplasms; FHIT; C-erbB-2; immunohistochemistry 脆性组氨酸三联体基因（fragile histidine triad gene,FHIT）是1996年Ohta等用定位克隆及外显子捕获技术在3p14·2首先克隆出来的,在多种肿瘤中,均有改变,以缺失为主。目前认为, FHIT基因是一个候选的抑癌基因。C-erbB-2已证实是一个