FasL受体在突出腰椎间盘髓核细胞中表达及意义.docVIP

FasL受体在突出腰椎间盘髓核细胞中表达及意义 　【摘要】 目的：检测突出的腰椎间盘髓核中FasL受体的表达情况，探讨其与腰椎间盘突出症发生与发展的关系。方法：根据美国矫形外科学会（AAOS）及国际腰椎研究会（ISSLS）分类，将20例术中取出的腰椎间盘突出症患者髓核标本分成退变组（10例）、脱出组（10例），并以7例外伤性脑死亡青年患者作为正常对照组，应用免疫组化法检测髓核中FasL受体表达情况并通过电镜查找凋亡细胞。结果：（1）FasL受体主要定位于细胞膜，呈均匀的棕黄色细颗粒状或粗细不等；（2）3组FasL阳性表达率明显不同（Plt;0.05）；（3）Fas阳性表达率的高低同年龄有关（r=0.4102，Plt;0.05），但与性别及病程无关；（4）电镜可观测到细胞凋亡的一些形态学变化。结论：腰椎间盘、突出髓核细胞中存在FasL受体的表达，突出类型不同，FasL受体表达的程度也不同，其异常表达诱导了髓核细胞的凋亡，可能在腰椎间盘突出症的发病过程中起着重要作用。 【关键词】 腰椎间盘突出症；髓核细胞；凋亡；FasL；免疫组织化学 [Abstract] Objective: To investigate the cell type that expressed gene proteins FasL and the evidence of apoptosis of the disc cells in herniated lumbar disc tissues. Methods: Twenty herniated lumbar disc tissues(contained disc n=10 and noncontained disc n=10)and seven normal disc tissues were examined to investigate the cell type that expressed FasL and any evidence of apoptosis of the disc cells by using immunohistochemical S-P method and under electron microscope respectively. The percentage of FasL-positive disc cells was calculated and compared with clinical and radiologic data.Results:（1）FasL was expressed in the cell membrane of the disc cells.（2）There was a significant difference in the percentages of FasL-positive disc cells among the contained, noncontained and normal discs(Plt;0.05).（3）The percentages of FasL-positive disc cells correlated significantly with the patient’s ages(r=0.4102，Plt;0.05)， but not with the sex,duration of sciatica(Pgt;0.05). There was some evidence of apoptosis of the disc cells under electron microscope.Conclusion: The expression of FasL on disc cells in herniated disc tissue is identified. The current results indicate that the abnormal expression of FasL on disc cells may lead to FasL-mediated apoptosis. The degrees of expression of FasL are varied depending on the types of herniation. [Key words] Protrusion of intervertebral disc; Apoptosis disc cell; FasL receptor; Herniated lumbar disc; Immunohistochemistry腰椎间盘突出症是腰腿痛最常见的原因之一，退行性变是基本致病因素，但其机理并不完全清楚，有证据表明，凋亡在椎间