有哪些信誉好的足球投注网站cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with salmonella enteritidis细胞因子信号在脾静脉注射后白细胞从接种疫苗和未接种鸡感染肠炎沙门氏菌.pdfVIP
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cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with salmonella enteritidis细胞因子信号在脾静脉注射后白细胞从接种疫苗和未接种鸡感染肠炎沙门氏菌
Cytokine Signaling in Splenic Leukocytes from Vaccinated and Non-Vaccinated Chickens after Intravenous Infection with Salmonella Enteritidis Marta Matulova, Hana Stepanova, Frantisek Sisak, Hana Havlickova, Marcela Faldynova, Kamila Kyrova, Jiri Volf, Ivan Rychlik* Veterinary Research Institute, Brno, Czech Republic Abstract In order to design a new Salmonella enterica vaccine, one needs to understand how naive and immune chickens interact differently when exposed to S. enterica. In this study we therefore determined the immune response of vaccinated and non- vaccinated chickens after intravenous infection with Salmonella enterica serovar Enteritidis (S. Enteritidis). Using flow cytometry we showed that 4 days post infection (DPI), counts of CD4 and B-lymphocytes did not change, CD8 and cd T- lymphocytes decreased and macrophages and heterophils increased in the spleen. When vaccinated and non-vaccinated chickens were compared, only macrophages and heterophils were found in significantly higher counts in the spleens of the non-vaccinated chickens. The non-vaccinated chickens also expressed higher anti-LPS antibodies than the vaccinated chickens. The expression of interleukin (IL)1b, IL6, IL8, IL18, LITAF, IFNc and iNOS did not exhibit any clear pattern in the cells sorted from the spleens of vaccinated or non-vaccinated chickens. Only IL17 and IL22 showed a differential expression in the CD4 T-lymphocytes of the vaccinated and non-vaccinated chickens at 4 DPI, both being expressed at a higher level in the non-vaccinated chickens. Due to a similar IFNc expression in the CD4 T-lymphocytes in both the vaccinated and non- vaccinated chickens, and a variable IL17 expression oscillating around IFNc expression levels, the IL17:IFNc ratio in CD4 T- lymphocytes was found to be central for the outcome of the immune response. When IL17 was expresse
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