cytomegalovirus micrornas facilitate persistent virus infection in salivary glands巨细胞病毒小分子核糖核酸促进唾液腺持续的病毒感染.pdfVIP

Cytomegalovirus microRNAs Facilitate Persistent Virus Infection in Salivary Glands ¨ 1. 2. 1 3 ´ 3 1 Lars Dolken , Astrid Krmpotic , Sheila Kothe , Lee Tuddenham , Melanie Tanguy , Lisa Marcinowski , 1 4 4 4 1 2 Zsolt Ruzsics , Naama Elefant , Yael Altuvia , Hanah Margalit , Ulrich H. Koszinowski , Stipan Jonjic *, ´ 3* Sebastien Pfeffer 1 Max von Pettenkofer-Institute, Ludwig-Maximilians-University Munich, Munich, Germany, 2 Department of Histology and Embryology, Faculty of Medicine University of ´ ´ ´ ´ Rijeka, Rijeka, Croatia, 3 Architecture et Reactivite de l’ARN, Universite de Strasbourg, Institut de Biologie Moleculaire et Cellulaire du CNRS, Strasbourg, France, 4 Department of Microbiology and Molecular Genetics, Faculty of Medicine, The Hebrew University, Jerusalem, Israel Abstract Micro (mi)RNAs are small non-coding RNAs that regulate the expression of their targets’ messenger RNAs through both translational inhibition and regulation of target RNA stability. Recently, a number of viruses, particularly of the herpesvirus family, have been shown to express their own miRNAs to control both viral and cellular transcripts. Although some targets of viral miRNAs are known, their function in a physiologically relevant infection remains to be elucidated. As such, no in vivo phenotype of a viral miRNA knock-out mutant has been described so far. Here, we report on the first functional phenotype of a miRNA knock-out virus in v