critical role of perforin-dependent cd8+ t cell immunity for rapid protective vaccination in a murine model for human smallpox关键作用perforin-dependent cd8 + t细胞免疫的快速保护人类天花疫苗接种小鼠模型.pdfVIP

Critical Role of Perforin-dependent CD8+ T Cell Immunity for Rapid Protective Vaccination in a Murine Model for Human Smallpox 1. 2. 1. 3 4 Melanie Kremer , Yasemin Suezer , Asisa Volz , Theresa Frenz , Monir Majzoub , Kay-Martin 2 1 3 1 Hanschmann , Michael H. Lehmann , Ulrich Kalinke , Gerd Sutter * 1 Institute for Infectious Diseases and Zoonoses, University of Munich LMU, Muenchen, Germany, 2 Paul-Ehrlich-Institut, Langen, Germany, 3 Institute for Experimental Infection Research, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Helmholtz Centre for Infection Research, Braunschweig, and Hannover Medical School, Hannover, Germany, 4 Institute of Veterinary Pathology, University of Munich LMU, Muenchen, Germany Abstract Vaccination is highly effective in preventing various infectious diseases, whereas the constant threat of new emerging pathogens necessitates the development of innovative vaccination principles that also confer rapid protection in a case of emergency. Although increasing evidence points to T cell immunity playing a critical role in vaccination against viral diseases, vaccine efficacy is mostly associated with the induction of antibody responses. Here we analyze the immunological mechanism(s) of rapidly protective vaccinia virus immunization using mousepox as surrogate model for human smallpox. We found that fast protection against lethal systemic poxvirus disease solely depended on CD4 and CD8 T cell responses induced by vaccination with highly atte