conjunctiva-associated lymphoid tissue (calt) reactions to antiglaucoma prostaglandins with or without bak-preservative in rabbit acute toxicity studyconjunctiva-associated淋巴组织(calt)反应antiglaucoma前列腺素有或没有bak-preservative在兔急性毒性研究.pdfVIP

Conjunctiva-Associated Lymphoid Tissue (CALT) Reactions to Antiglaucoma Prostaglandins with or without BAK-Preservative in Rabbit Acute Toxicity Study Hong Liang1,2,3,4, Christophe Baudouin1,2,3,4,5,6, Antoine Labbe1,2,3,4,6, Luisa Riancho1,2,3, Franc¸oise Brignole-Baudouin1,2,3,4,5,7* 1 INSERM, U968, Paris, France, 2 UPMC Univ Paris 06, UMR_S 968, Institut de la Vision, Paris, France, 3 CNRS, UMR_7210, Paris, France, 4 Centre Hospitalier National ˆ ˆ ´ d’Ophtalmologie des Quinze-Vingts, INSERM-DHOS CIC 503, Paris, France, 5 Assistance Publique - Hopitaux de Paris Hopital Ambroise Pare, Service d’Ophtalmologie, ´ ´ ´ Boulogne-Billancourt, France, 6 Universite Versailles Saint-Quentin-en-Yvelines, Versailles, France, 7 Universite Paris Descartes, Faculte des Sciences Pharmaceutiques et Biologiques, Laboratoire de Toxicologie, Paris, France Abstract Conjunctiva-associated lymphoid tissue (CALT) is closely associated with ocular surface immunity. This study investigated the effects of antiglaucoma prostaglandin analogs with or without benzalkonium chloride (BAK) preservative on organized CALT using an acute toxic model. A total of 48 albino rabbits were used and seven groups of treatments were constituted. Solutions (50 ml) of PBS, 0.02%BAK, 0.02%BAK+ latanoprost, 0.015%BAK+travoprost, 0.005%BAK+ bimatoprost, BAK-freetravoprost preserved with the SofZiaH system or BAK-freetafluprost were instilled 15 times at 5-min intervals in both eyes. CALT changes were analyzed using in vivo confocal microscopy (IVCM), immunohistology in cryosections for detecting