correlation coefficients between different methods of expressing bacterial quantification using real time pcr不同方法之间的相关系数表达细菌使用实时pcr量化.pdfVIP

Int. J. Mol. Sci. 2012, 13, 2119-2132; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Correlation Coefficients Between Different Methods of Expressing Bacterial Quantification Using Real Time PCR Bahman Navidshad 1,2, Juan Boo Liang 2,* and Mohammad Faseleh Jahromi 3 1 Department of Animal Science, University of Mohaghegh Ardabili, 56199-11367, Ardabil, Iran; E-Mail: bnavidshad@uma.ac.ir 2 Institute of Tropical Agriculture, University Putra Malaysia, 43400 UPM Serdang, Malaysia 3 Laboratory of Industrial Biotechnology, Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Malaysia; E-Mail: mohammadfj2006@ * Author to whom correspondence should be addressed; E-Mail: jbliang@.my; Tel.: +60-3-8947-2132; Fax: +60-3-8947-2213. Received: 30 January 2012 / Accepted: 2 February 2012 / Published: 16 February 2012 Abstract: The applications of conventional culture-dependent assays to quantify bacteria populations are limited by their dependence on the inconsistent success of the different culture-steps involved. In addition, some bacteria can be pathogenic or a source of endotoxins and pose a health risk to the researchers. Bacterial quantification based on the real-time PCR method can overcome the above-mentioned problems. However, the quantification of bacteria using this approach is com