correlation index-based responsible-enzyme gene screening (cires), a novel dna microarray-based method for enzyme gene involved in glycan biosynthesis相关基于索引responsible-enzyme基因筛查(cires),一种新的dna芯片酶和多糖生物合成有关的基因的方法.pdfVIP

Correlation Index-Based Responsible-Enzyme Gene Screening (CIRES), a Novel DNA Microarray-Based Method for Enzyme Gene Involved in Glycan Biosynthesis 1,4 1,5 4 1,5 2 3 4 Harumi Yamamoto , Hiromu Takematsu *, Reiko Fujinawa , Yuko Naito , Yasushi Okuno , Gozoh Tsujimoto , Akemi Suzuki , Yasunori Kozutsumi1,4,5 1 Laboratory of Membrane Biochemistry and Biophysics, Graduate School of Biostudies, Kyoto University, Sakyo, Kyoto, Japan, 2 Department of PharmacoInformatics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto, Japan, 3 Department of Genomic Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto, Japan, 4 Supra-Biomolecular System Research Group, RIKEN Frontier Research System, RIKEN, Wako, Saitama, Japan, 5 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST), Kawaguchi, Saitama, Japan Background. Glycan biosynthesis occurs though a multi-step process that requires a variety of enzymes ranging from glycosyltransferases to those involved in cytosolic sugar metabolism. In many cases, glycan biosynthesis follows a glycan- specific, linear pathway. As glycosyltransferases are generally regulated at the level of transcription, assessing the overall transcriptional profile for glycan biosynthesis genes seems warranted. However, a systematic approach for assessing the correlation between glycan expression and glycan-related gene expression has not been reported previously. Methodology. To facilitate genetic analysis of glycan biosynthesis, we sought to correlate the expression of genes involved in cell-surface glycan formation with the expression of the glycan