connector inversion probe technology a powerful one-primer multiplex dna amplification system for numerous scientific applications连接器反演探针技术的强大one-primer多路复用dna扩增系统众多的科学应用.pdfVIP

Connector Inversion Probe Technology: A Powerful One- Primer Multiplex DNA Amplification System for Numerous Scientific Applications 1,2 3 1 ˚ ´ 2 1 4,5 1,6 Michael S. Akhras , Magnus Unemo , Sreedevi Thiyagarajan , Pal Nyren , Ronald W. Davis , Andrew Z. Fire , Nader Pourmand * 1 Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America, 2 Department of Biotechnology, Royal ¨ Institute of Technology, Stockholm, Sweden, 3 National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro ¨ University Hospital, Orebro, Sweden, 4 Department of Pathology, Stanford University School of Medicine, Stanford, California, United States of America, 5 Department of Genetics, Stanford University School of Medicine, Stanford, California, United States of America, 6 Biomolecular Engineering, University of California at Santa Cruz, Santa Cruz, California, United States of America We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system. As a proof-of-concept, this novel method, which employs a Connector Inversion Probe (CIPer), was tested as a genetic tool for pathogen diagnosis, typing, and antibiotic resistance screening with two distinct systems: i) a conserved sequence primer system for genotyping Human Papillomavirus (HPV), a cancer-associated viral agent and ii) screening for antibiotic resistance mutations in the bacterial