comparative genomics suggests that the fungal pathogen pneumocystis is an obligate parasite scavenging amino acids from its hosts lungs比较基因组学表明,肺孢子菌是专性寄生真菌病原体清除氨基酸从其宿主肺.pdfVIP

Comparative Genomics Suggests that the Fungal Pathogen Pneumocystis Is an Obligate Parasite Scavenging Amino Acids from Its Host’s Lungs 1 ´ ´ 1,2¤ ´ 1 3 ´4 Philippe M. Hauser *, Frederic X. Burdet , Ousmane H. Cisse , Laurent Keller , Patrick Taffe , Dominique Sanglard1, Marco Pagni2 1 Institute of Microbiology, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland, 2 Vital-IT Group, Swiss Institute of Bioinformatics, ´ ´ ´ Lausanne, Switzerland, 3 Departement d’Ecologie et Evolution, University of Lausanne, Lausanne, Switzerland, 4 Data Center, Swiss HIV Cohort Study, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland Abstract Pneumocystis jirovecii is a fungus causing severe pneumonia in immuno-compromised patients. Progress in understanding its pathogenicity and epidemiology has been hampered by the lack of a long-term in vitro culture method. Obligate parasitism of this pathogen has been suggested on the basis of various features but remains controversial. We analysed the 7.0 Mb draft genome sequence of the closely related species Pneumocystis carinii infecting rats, which is a well established experimental model of the disease. We predicted 8’085 (redundant) peptides and 14.9% of them were mapped onto the KEGG biochemical pathways. The proteome of the closely related yeast Schizosaccharomyces pombe was used as a control for the annotation procedure (4’974 genes, 14.1% mapped). About two thirds of the mapped peptides of each organism (65.7% and 73.2%, respectively) corresponded to crucial enzymes for the basal metabolism and standard cellular processes.