computational identification of four spliceosomal snrnas from the deep-branching eukaryote giardia intestinalis计算识别四个spliceosomal deep-branching真核鞭毛虫intestinalis核内小rna.pdfVIP

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computational identification of four spliceosomal snrnas from the deep-branching eukaryote giardia intestinalis计算识别四个spliceosomal deep-branching真核鞭毛虫intestinalis核内小rna.pdf

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computational identification of four spliceosomal snrnas from the deep-branching eukaryote giardia intestinalis计算识别四个spliceosomal deep-branching真核鞭毛虫intestinalis核内小rna

Computational Identification of Four Spliceosomal snRNAs from the Deep-Branching Eukaryote Giardia intestinalis Xiaowei Sylvia Chen*, W. Timothy J. White, Lesley J. Collins, David Penny Allan Wilson Centre for Molecular Ecology and Evolution, IMBS, Massey University, Palmerston North, New Zealand Abstract RNAs processing other RNAs is very general in eukaryotes, but is not clear to what extent it is ancestral to eukaryotes. Here we focus on pre-mRNA splicing, one of the most important RNA-processing mechanisms in eukaryotes. In most eukaryotes splicing is predominantly catalysed by the major spliceosome complex, which consists of five uridine-rich small nuclear RNAs (U-snRNAs) and over 200 proteins in humans. Three major spliceosomal introns have been found experimentally in Giardia; one Giardia U-snRNA (U5) and a number of spliceosomal proteins have also been identified. However, because of the low sequence similarity between the Giardia ncRNAs and those of other eukaryotes, the other U-snRNAs of Giardia had not been found. Using two computational methods, candidates for Giardia U1, U2, U4 and U6 snRNAs were identified in this study and shown by RT-PCR to be expressed. We found that identifying a U2 candidate helped identify U6 and U4 based on interactions between them. Secondary structural modelling of the Giardia U-snRNA candidates revealed typical features of eukaryotic U-snRNAs. We demonstrate a successful approach to combine computational and experimental methods to identify expected ncRNAs in a highly divergent protist genome. Our findings reinforce the conclusion that spliceosomal small-nuclear RNAs existed in the last common ancestor of eukaryotes. Citation: Chen XS, White WTJ, Collins LJ, Penny D (2008) Computational Identification of Four Spliceosomal snRNAs from the Deep-Branching Eukaryote Giardia intestinalis. PLoS ONE 3(8): e3106.