computational analysis of the spatiotemporal coordination of polarized pi3k and rac1 activities in micro-patterned live cells计算分析的时空协调极化pi3k和rac1活动micro-patterned活细胞.pdfVIP

computational analysis of the spatiotemporal coordination of polarized pi3k and rac1 activities in micro-patterned live cells计算分析的时空协调极化pi3k和rac1活动micro-patterned活细胞.pdf

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computational analysis of the spatiotemporal coordination of polarized pi3k and rac1 activities in micro-patterned live cells计算分析的时空协调极化pi3k和rac1活动micro-patterned活细胞

Computational Analysis of the Spatiotemporal Coordination of Polarized PI3K and Rac1 Activities in Micro-Patterned Live Cells 1. 2. 3 1 2 1,5 Shaoying Lu , Tae-jin Kim , Chih-En Chen , Mingxing Ouyang , Jihye Seong , Xiaoling Liao , Yingxiao Wang1,2,3,4* 1 Department of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, Illinois, United States of America, 2 Neuroscience Program, University of Illinois at Urbana-Champaign, Champaign, Illinois, United States of America, 3 Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign, Champaign, Illinois, United States of America, 4 Department of Molecular and Integrative Physiology, Center of Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Champaign, Illinois, United States of America, 5 Institute of Biomaterials and Living Cell Imaging Technology, Chongqing University of Science and Technology, Chongqing, People’s Republic of China Abstract Polarized molecular activities play important roles in guiding the cell toward persistent and directional migration. In this study, the polarized distributions of the activities of phosphatidylinositol 3-kinase (PI3K) and the Rac1 small GTPase were monitored using chimeric fluorescent proteins (FPs) in cells constrained on micro-patterned strips, with one end connecting to a neighboring cell (junction end) and the other end free of cell-cell contact (free end). The recorded spatiotemporal dynamics of the fluorescent intensity from different cells was scaled into a uniform coordinate system and applied to compute the molecular activity landscapes in spa

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