comparison of functional proteomic analyses of human breast cancer cell lines t47d and mcf7功能的比较蛋白质组学分析人类乳腺癌细胞系t47d和mcf7.pdfVIP

Comparison of Functional Proteomic Analyses of Human Breast Cancer Cell Lines T47D and MCF7 Juliette Adjo Aka1,2, Sheng-Xiang Lin1,2* ´ ´ 1 Laboratory of Endocrinology and Genomics, CHUL Research Center (CHUQ), Laval University, Quebec City, Quebec, Canada, 2 Department of Molecular Medicine, ´ ´ Faculty of Medicine, Laval University, Quebec City, Quebec, Canada Abstract T47D and MCF7 are two human hormone-dependent breast cancer cell lines which are widely used as experimental models for in vitro and in vivo (tumor xenografts) breast cancer studies. Several proteins involved in cancer development were identified in these cell lines by proteomic analyses. Although these studies reported the proteomic profiles of each cell line, until now, their differential protein expression profiles have not been established. Here, we used two-dimensional gel and mass spectrometry analyses to compare the proteomic profiles of the two cell lines, T47D and MCF7. Our data revealed that more than 164 proteins are differentially expressed between them. According to their biological functions, the results showed that proteins involved in cell growth stimulation, anti-apoptosis mechanisms and cancerogenesis are more strongly expressed in T47D than in MCF7. These proteins include G1/S-specific cyclin-D3 and prohibitin. Proteins implicated in transcription repression and apoptosis regulation, including transcriptional repressor NF-X1, nitrilase homolog 2 and interleukin-10, are, on the contrary, more strongly expressed in MCF7 as compared to T47D. Five proteins that were previously described as breast cancer biomarkers, namely catheps