comparison of influenza and siv specific cd8 t cell responses in macaques比较的流感,在猕猴猴免疫缺陷病毒特异性cd8 t细胞反应.pdfVIP

Comparison of Influenza and SIV Specific CD8 T Cell Responses in Macaques 1 1 1 1,2 1 Sinthujan Jegaskanda , Jeanette C. Reece , Robert De Rose , John Stambas , Lucy Sullivan , Andrew G. 1 1 . 1. Brooks , Stephen J. Kent * , Amy Sexton 1 Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia, 2 School of Medicine, Deakin University, Waurn Ponds, Victoria, Australia Abstract Macaques are a potentially useful non-human primate model to compare memory T-cell immunity to acute virus pathogens such as influenza virus and effector T-cell responses to chronic viral pathogens such as SIV. However, immunological reagents to study influenza CD8+ T-cell responses in the macaque model are limited. We recently developed an influenza- SIV vaccination model of pigtail macaques (Macaca nemestrina) and used this to study both influenza-specific and SIV- specific CD8+ T-cells in 39 pigtail macaques expressing the common Mane-A*10+ (Mane-A01*084) MHC-I allele. To perform comparative studies between influenza and SIV responses a common influenza nucleoprotein-specific CD8+ T-cell response was mapped to a minimal epitope (termed RA9), MHC-restricted to Mane-A*10 and an MHC tetramer developed to study this response. Influenza-specific memory CD8+ T-cell response maintained a highly functional profile in terms of multitude of effector molecule expression (CD107a, IFN-c, TNF-a, MIP-1 b and IL-2) and showed high avidity even in the setting of SIV infection. In contrast, within weeks following active SIV infection, SIV-