comparative analysis of plasmids in the genus listeria属李斯特菌的质粒进行比较分析.pdfVIP

Comparative Analysis of Plasmids in the Genus Listeria 1 2 1 3 3 Carsten Kuenne , Sonja Voget , Jordan Pischimarov , Sebastian Oehm , Alexander Goesmann , Rolf 2 1 1 Daniel , Torsten Hain *, Trinad Chakraborty * 1 Institute of Medical Microbiology, Justus-Liebig University, Giessen, Germany, 2 Goettingen Genomics Laboratory, Institute for Microbiology and Genetics, Georg-August University Goettingen, Goettingen, Germany, 3 Bioinformatics Resource Facility, Center for Biotechnology, Bielefeld University, Bielefeld, Germany Abstract Background: We sequenced four plasmids of the genus Listeria, including two novel plasmids from L. monocytogenes serotype 1/2c and 7 strains as well as one from the species L. grayi. A comparative analysis in conjunction with 10 published Listeria plasmids revealed a common evolutionary background. Principal Findings: All analysed plasmids share a common replicon-type related to theta-replicating plasmid pAMbeta1. Nonetheless plasmids could be broadly divided into two distinct groups based on replicon diversity and the genetic content of the respective plasmid groups. Listeria plasmids are characterized by the presence of a large number of diverse mobile genetic elements and a commonly occurring translesion DNA polymerase both of which have probably contributed to the evolution of these plasmids. We detected small non-coding RNAs on some plasmids that were homologous to those present on the chromosome of L. monocytogenes EGD-e. Multiple genes involved in heavy metal resistance (cadmium, copper, arsenite) as well as multidrug efflux (MDR, SMR, MATE) were detected on all listerial plasmids. These factors pr