communication between corneal epithelial cells and trigeminal neurons is facilitated by purinergic (p2) and glutamatergic receptors角膜上皮细胞和三叉神经元之间的通信是通过purinergic(p2)和glutamatergic受体.pdfVIP

Communication between Corneal Epithelial Cells and Trigeminal Neurons Is Facilitated by Purinergic (P2) and Glutamatergic Receptors 1. 1. 2 2 1 1 Duane J. Oswald , Albert Lee , Monique Trinidad , Cheryl Chi , Ruiyi Ren , Celeste B. Rich , Vickery Trinkaus-Randall1,2* 1 Departments of Biochemistry and Ophthalmology, Boston University School of Medicine, Boston, Massachusetts, United States of America, 2 Boston University School of Medicine, Boston, Massachusetts, United States of America Abstract Previously, we demonstrated that nucleotides released upon mechanical injury to corneal epithelium activate purinergic (P2) receptors resulting in mobilization of a Ca2+ wave. However, the tissue is extensively innervated and communication between epithelium and neurons is critical and not well understood. Therefore, we developed a co-culture of primary trigeminal neurons and human corneal limbal epithelial cells. We demonstrated that trigeminal neurons expressed a repertoire of P2Yand P2X receptor transcripts and responded to P2 agonists in a concentration-dependent manner. Mechanical injuries to epithelia in the co-cultures elicited a Ca2+ wave that mobilized to neurons and was attenuated by Apyrase, an ectonucleotidase. To elucidate the role of factors released from each cell type, epithelial and neuronal cells were cultured, injured, and the wound media from one cell type was collected and added to the other cell type. Epithelial wound media generated a rapid Ca2+ mobilization in neuronal cells that was abrogated in the presence of Apyrase, while neuronal wound media elicited a complex response in epithelial cells. The rapid Ca2+ mobilization was