blood glutathione s-transferase-π as a time indicator of stroke onset血谷胱甘肽s-transferase-π中风发病的时间指标.pdfVIP

Blood Glutathione S-Transferase-p as a Time Indicator of Stroke Onset 1 1,2 1 1 1 Natacha Turck *, Xavier Robin , Nadia Walter , Catherine Fouda , Alexandre Hainard , 3 3 1 4 3 Roman Sztajzel , Ghislaine Wagner , Denis F. Hochstrasser , Joan Montaner , Pierre R. Burkhard , Jean- Charles Sanchez1 1 Biomedical Proteomics Research Group, Department of Human Protein Sciences, Faculty of Medicine, Geneva, Switzerland, 2 Swiss Institute of Bioinformatics, Medical University Centre, Geneva, Switzerland, 3 Department of Neurology, Geneva University Hospitals and Faculty of Medicine, University of Geneva, Geneva, Switzerland, 4 Neurovascular Research Laboratory, VHIR Stroke Unit, Vall d’Hebron Hospital, Autonomous University of Barcelona (UAB), Catalonia, Spain Abstract Background: Ability to accurately determine time of stroke onset remains challenging. We hypothesized that an early biomarker characterized by a rapid increase in blood after stroke onset may help defining better the time window during which an acute stroke patient may be candidate for intravenous thrombolysis or other intravascular procedures. Methods: The blood level of 29 proteins was measured by immunoassays on a prospective cohort of stroke patients (N = 103) and controls (N = 132). Mann-Whitney U tests, ROC curves and diagnostic odds ratios were applied to evaluate their clinical performances. Results: Among the 29 molecules tested, GST-p concentration was the most significantly elevated marker in the blood of stroke patients (p,0.001). More importantly, GST-p displayed the best area und