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biosafety of recombinant and wild type nucleopolyhedroviruses as bioinsecticides生物安全的重组和野生型nucleopolyhedroviruses bioinsecticides.pdf

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biosafety of recombinant and wild type nucleopolyhedroviruses as bioinsecticides生物安全的重组和野生型nucleopolyhedroviruses bioinsecticides

Int. J. Environ. Res. Public Health 2007, 4(2), 111-125 International Journal of Environmental Research and Public Health ISSN 1661-7827 © 2007 by MDPI Biosafety of Recombinant and Wild Type Nucleopolyhedroviruses as Bioinsecticides 1* 1 1 1 2 Mohamed-Bassem Ashour , Didair A. Ragheb , El-Sayed A. El-Sheikh , El-Adarosy A. Gomaa , Shizuo G. Kamita , 2 and Bruce D. Hammock 1Department of Plant Protection, Faculty of Agriculture, Zagazig University, Zagazig, Egypt 2Department of Entomology and Cancer Research Center, University of California, Davis, CA 95616, USA *Correspondence to Dr. Mohamed-Bassem Ashour. Email: bassemashour@ Received: 23 January 2007 / Accepted: 30 April 2007 / Published: 30 June 2007 Abstract: The entomopathogenic Autographa californica (Speyer) nucleopolyhedrovirus (AcMNPV) has been genetically modified to increase its speed of kill. The potential adverse effects of a recombinant AcMNPV (AcAaIT) as well as wild type AcMNPV and wild type Spodoptera littoralis NPV (SlNPV) were studied. Cotton plants were treated with these viruses at concentrations that were adjusted to resemble the recommended field application rate (4 x 1012