bioinformatic and genetic association analysis of microrna target sites in one-carbon metabolism genes生物信息学和基因关联分析微目标站点一个碳代谢的基因.pdfVIP

Bioinformatic and Genetic Association Analysis of MicroRNA Target Sites in One-Carbon Metabolism Genes 1¤ 1 2 3 2 4 Nicole Stone , Faith Pangilinan , Anne M. Molloy , Barry Shane , John M. Scott , Per Magne Ueland , 5 6 1 1 James L. Mills , Peader N. Kirke , Praveen Sethupathy *, Lawrence C. Brody * 1 Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America, 2 School of Immunology and Biochemistry, Trinity College, Dublin, Ireland, 3 Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California, United States of America, 4 Section of Pharmacology, Institute of Medicine, University of Bergen and Haukeland University Hospital, Bergen, Norway, 5 Division of Epidemiology, Statistics, and Prevention Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America, 6 Child Health Epidemiology Unit, Health Research Board of Ireland, Dublin, Ireland Abstract One-carbon metabolism (OCM) is linked to DNA synthesis and methylation, amino acid metabolism and cell proliferation. OCM dysfunction has been associated with increased risk for various diseases, including cancer and neural tube defects. MicroRNAs (miRNAs) are ,22 nt RNA regulators that have been implicated in a wide array of basic cellular processes, such as differentiation and metabolism. Accordingly, mis-regulation of miRNA expression and/or activity can underlie complex disease etiology. We examined the possibility of OCM regulation by miRNAs. Using comp