xenogeneic graft-versus-host-disease in nod-scid il-2rγnull mice display a t-effector memory phenotype异种的移植物抗宿主病在nod-scid il-2rγnull老鼠显示t效应记忆表型.pdfVIP

Xenogeneic Graft-versus-Host-Disease in NOD-scid IL- 2Rcnull Mice Display a T-Effector Memory Phenotype 1,2 1 1¤ 2 3 Niwa Ali , Barry Flutter , Robert Sanchez Rodriguez , Ehsan Sharif-Paghaleh , Linda D. Barber , 2 1 Giovanna Lombardi , Frank O. Nestle * 1 St. John’s Institute of Dermatology, King’s College London and NIHR Biomedical Research Centre, London, United Kingdom, 2 MRC Centre for Transplantation, King’s College London and NIHR Biomedical Research Centre, London, United Kingdom, 3 Department of Haematological Medicine, King’s College London, London, United Kingdom Abstract The occurrence of Graft-versus-Host Disease (GvHD) is a prevalent and potentially lethal complication that develops following hematopoietic stem cell transplantation. Humanized mouse models of xenogeneic-GvHD based upon immunodeficient strains injected with human peripheral blood mononuclear cells (PBMC; ‘‘Hu-PBMC mice’’) are important tools to study human immune function in vivo. The recent introduction of targeted deletions at the interleukin-2 common gamma chain (IL-2Rcnull), notably the NOD-scid IL-2Rcnull (NSG) and BALB/c-Rag2null IL-2Rcnull (BRG) mice, has led to improved human cell engraftment. Despite their widespread use, a comprehensive characterisation of engraftment and GvHD development in the Hu-PBMC NSG and BRG models has never been performed in parallel. We compared engrafted human lymphocyte populations in the peripheral blood, spleens, lymph nodes and bone marrow of thes