willow leaves extracts contain anti-tumor agents effective against three cell types柳树的叶子提取物含有抗肿瘤药物有效对三种细胞类型.pdfVIP

Willow Leaves’ Extracts Contain Anti-Tumor Agents Effective against Three Cell Types 1,3 1 2 3 Hany A. El-Shemy *, Ahmed M. Aboul-Enein , Khalid Mostafa Aboul-Enein , Kounosuke Fujita 1 Department of Biochemistry, Faculty of Agriculture, Cairo University, Giza, Egypt, 2 Department of Clinical Pathology, National Cancer Institute, Cairo University, Cairo, Egypt, 3 Graduate School of Biosphere Sciences, Hiroshima University, Hiroshima, Japan Many higher plants contain novel metabolites with antimicrobial, antifungal and antiviral properties. However, in the developed world almost all clinically used chemotherapeutics have been produced by in vitro chemical synthesis. Exceptions, like taxol and vincristine, were structurally complex metabolites that were difficult to synthesize in vitro. Many non-natural, synthetic drugs cause severe side effects that were not acceptable except as treatments of last resort for terminal diseases such as cancer. The metabolites discovered in medicinal plants may avoid the side effect of synthetic drugs, because they must accumulate within living cells. The aim here was to test an aqueous extract from the young developing leaves of willow (Salix safsaf, Salicaceae) trees for activity against human carcinoma cells in vivo and in vitro. In vivo Ehrlich Ascites Carcinoma Cells (EACC) were injected into the intraperitoneal cavity of mice. The willow extract was fed via stomach tube. The (EACC) derived tumor growth was reduced by the willow extract and death was delayed (for 35 days). In vitro the willow extract could kill the majority (75%–80%) of abnormal cells among primary cells harvested from seven patients with acute lymphoblastic leukemia (ALL) and 13 with AML (acute myeloid leukemia). DNA fragmentation patterns within treated cells inferred targeted cell death by