单味中药黄芪改善胰岛素抵抗对血尿酸影响相关性探究.docVIP

单味中药黄芪改善胰岛素抵抗对血尿酸影响相关性探究 　 作者：杨传经　李雪梅　孔德明　赵胜 【摘要】 目的研究单味中药黄芪改善胰岛素抵抗(IR)对血尿酸的影响。方法选用 60只成年Wistar雄性大鼠，随机分成正常对照组（12只）和模型组（48只），模型组给予高脂高糖饲料喂养6周，6周后再用腺嘌呤〔100 mg/(kg·d）〕加乙胺丁醇〔250 mg/(kg·d）〕灌胃2周，造成胰岛素抵抗并高尿酸血症的大鼠模型。将造模成功的大鼠随机分成黄芪治疗组、别嘌醇治疗组、黄芪加别嘌醇治疗组、模型对照组进行实验，分别于治疗的第3周、第6周测量空腹血糖（FPG）、空腹胰岛素（FINS）、血尿酸（UA）及测算胰岛素敏感指数（ISI）等指标，观察其对胰岛素抵抗并高尿酸血症的影响。结果与模型对照组比较，中药黄芪明显降低IR并高尿酸血症大鼠的FPG、FINS、UA（P＜0.05或P＜0.01），增加ISI（P＜0.01）；与模型对照组比较，中药黄芪与别嘌醇合用在改善IR的同时降低UA最明显（P＜0.001）；黄芪治疗组治疗后UA与FPG、FINS呈显著正相关，r分别为0.62和0.66（P＜0.05）；与ISI呈显著负相关,r为-0.64（P＜0.05）。结论中药黄芪具有胰岛素增敏作用；中药黄芪对IR并高尿酸血症模型大鼠具有明显降血尿酸作用；中药黄芪与别嘌醇合用在改善IR的同时降低血尿酸水平，其降UA的作用较单独使用黄芪或单独使用别嘌醇更明显；中药黄芪降低IR并高尿酸血症模型大鼠血尿酸的作用似与提高ISI及降低FPG、 FINS密切相关，因此，提示IR应是高尿酸血症、高血糖、高血压病等代谢性疾病发生的共同的重要的病理生理学基础。黄芪可能成为一个很好的治疗IR和IR并高尿酸血症的中药。 【关键词】 中药黄芪；胰岛素抵抗并高尿酸血症; 大鼠 　　Abstract:ObjectiveTo study the effects of the single Chinese traditional medicine of Radix astragali(RA) on insulin resistance and serum uric acid. MethodsSixty Wistar rats were divided into 2 groups randomly: normol group(n=12), and model group(n=48).The model rats were fed with high fat-sugar diet for 6 weeks, then fed with adenine(100 mg·kg-1·d-1) and ethambutol(250 mg·kg-1·d-1) for 2 weeks to induce the IR and hyperuricemia model. Fourty-eight successful model rats were divided into blank-model group,RA group,allopurinol group,RA+allopurinol group.The serum uric acid(UA), Fasting Plasma Glucose(FPG),Fasting Insulin(FINS) and Insulin Sensitivity Index(ISI) were detected in 3 and 6 weeks,and the relationship between UA level after RA and insulin resistance was analyzed.Results(1) RA could distinctly decrease FPG,FINS and UA levels of insulin resistance(IR)+hyperuricemia model rats (P＜0.05 vs P＜0.01）,and increase ISI level(P＜0.01）compared with blank-model group .(2) The effect of RA+allopurinol group was the most distinct to decrease UA level（P＜0.001）compare with blank-model group.(3) UA was in positive correlation with FPG and FINS after RA treatment (r=0.62,P＜0.05;r=0.66, P＜0.05）,and in negative correlati