单唾液酸神经节苷酯对兔视网膜缺血再灌注损伤保护作用.docVIP

单唾液酸神经节苷酯对兔视网膜缺血再灌注损伤保护作用 　 作者：赵春梅　岳向东　韩笑　赵志惠　景晓彬 【摘要】 　　目的：通过监测视网膜电流图（ERG）a，b波恢复的百分率，观察单唾液酸神经节苷酯（GM-1）球后注射给药在视网膜缺血再灌注损伤中的保护作用。方法：健康新西兰大耳白兔20只，随机分为实验组和治疗组， 用眼内灌注方法制成视网膜急性缺血的动物模型，治疗组于撤压前5min球后注射GM-1 （1mg/kg），实验组只加压不给药，均记录缺血前ERG，缺血期ERG及再灌注30,60,90min时的ERG图形。 结果：两组缺血前的 a，b波振幅无显著差异（P＞0.05）。a波振幅的恢复在再灌注30，60及90min两组间相比均无显著差异（P＞0.05）。b波振幅的恢复在再灌注30，60及90min两组间相比均有显著性的差异（两组间30，60及90min比较均P ＜0.01），治疗组均高于实验组。 结论：单唾液酸神经节苷酯对视网膜缺血再灌注损伤具有保护作用。 【关键词】 单唾液酸神经节苷酯　视网膜缺血再灌注　视网膜电流图 　　Protective effects of GM-1 in retinal ischemic injury Abstract AIM: To detect the recovery of amplitude a-wave and b-wave in reperfusion by using electroretinogram(ERG). The possible mechanism of effect of GM-1 through rerobulbar way in retinal ischemic injury was also observed. METHODS: Twenty Switzerland healthy rabbits with big ears were selected and randomly divided into experimental group and treated group. The rabbit’s retinal ischemic model was induced in intraocular pressure by perfusing the normal saline. The treated group was administered with GM-1(1mg/kg) as an intravenous push 5 minutes before the termination of ischemia. The experimental group was no medicine on it but pressure. The wave of ERG of the model eyes in the two groups were recorded respectively before and during ischemia and also at the 30, 60 and 90 minutes of reperfusion. RESULTS: The amplitude of a-wave and b- wave had no significance before ischemia (P gt;0.05). The a-wave amplitudes in the recovery between the two groups had no significance at 30, 60, and 90 minutes of reperfusion P gt;0.05). The b-wave amplitudes in the recovery in the treated group were higher than it in the experimental group. CONCLUSION: GM-1 has the protective effects in retinal ischemic injury induced by intraocular hypertension. · KEYWORDS: GM-1; retina ischemia injury; electroretinogram 　0引言 视网膜缺血是临床常见的一种综合征，如青光眼、视网膜中央血管阻塞、糖尿病性视网膜病变等，视网膜缺血性疾病是常见的引起视功能下降的病因。现有的资料认为，损伤的机制主要包括能量缺乏、钙离子超载、自由基的大量产生、兴奋毒性氨基酸的过量释放[1]、各种神经营养因子的缺乏及由此而启动了调控细胞凋亡的基因，使视网膜神经细胞因凋亡致死