前列腺癌组织EZH2基因表达及其意义.docVIP

前列腺癌组织EZH2基因表达及其意义 　 作者：宋力，崔小健，苏大军，谭国良 【关键词】 前列腺肿瘤；基因；EZH2 　　Expression profile and significance of EZH2 gene in primary prostate cance 　　【Abstract】 AIM: To determine the expression of EZH2 gene in prostate cancer （PCa） and to investigate the relation between EZH2 expression and biological characters of PCa. METHODS: In 40 cases of PCa, 20 cases of benign prostate hyperplasia (BPH), 20 cases of prostatic intraepithelial neoplasia (PIN), the expression of EZH2 was detected by semiquantitative RTPCR and the amplified product was sequenced. RESULTS: All 40 PCa cases were positive in EZH2 expression. There was no expression of EZH2 gene in BPH. Only one of the 20 PIN cases was seen EZH2 expressed positively. EZH2 expression was increased in the advanced compared with the primary PCa (P＜0.05), in the lowdifferentiated compared with the highlydifferentiated PCa (P＜0.05) and in the hormonedependent compared with the hormoneindependent PCa (P＜0.05). However, EZH2 expression did not have a linear correlation with the serum total prostate specific antigen level (P＞0.05). CONCLUSION: EZH2 gene plays an important role in the development of prostaic carcinoma. Therefore, the analysis of EZH2 expression will benefit the diagnosis and treatment of prostatic carcinoma. 　　【Keywords】 prostatic carcinoma; gene; EZH2 　　【摘要】 目的：探讨EZH2基因在列腺癌组织中的表达意义. 方法：采用半定量RT－PCR检测前列腺癌、前列腺上皮内瘤(PIN)，良性前列腺增生中EZH2表达状况并对其扩增产物进行测序. 结果：前列腺癌40例组织中，EZH2表达率（100％）. 良性前列腺增生组织无表达， PIN 20例中仅有1例有表达. 晚期前列腺癌组织表达明显高于早期前列腺癌（P＜0.05）；低分化前列腺癌表达高于中高分化前列腺癌（P＜0.05）；激素依赖性前列腺癌表达高于激素非依赖性前列腺癌（P＜0.05）. EZH2表达高低与前列腺血清总PSA浓度无关（P＞0.05）. 结论：EZH2基因表达状况与前列腺癌分期、分级有关，其表达对判断前列腺癌病理生物学特性和前列腺癌治疗有重要意义. 　　【关键词】 前列腺肿瘤；基因；EZH2 　　0引言 　　EZH2 (enhancer of zeste homolog 2)基因是最近识别的一种人类新基因，是PcG (polycomb group)基因家族的重要成员之一. 与前列腺癌的发生发展关系密切. 我们采用RTPCR方法研究EZH2在前列腺癌、前列腺上皮内瘤、良性前列腺增生中的表达状况，探讨其在前列腺癌发生发展中的作用及意义. 　　1对象和方法 　　1.1对象良性前列腺增生标本20例，年龄（66.5±10.2）岁；前列腺上皮内瘤标本20例，年龄(64.2±11.3）岁. 前列腺癌标本40例，年龄(67.5±12.2）岁. 按国际抗癌协会(UICC)的TNM分期标准, T1期9例、T2期8例、T3期11例、T4期12例，高分化