前列腺组织二氢睾酮结合蛋白及性激素作用探究.docVIP

前列腺组织二氢睾酮结合蛋白及性激素作用探究 　【摘要】 目的 测定前列腺组织中DHT结合蛋白的DHT结合容量，研究睾酮、雌二醇、孕酮对DHT结合容量的影响，初步分析DHT结合蛋白与性激素作用的性质。方法 20例正常前列腺组织，制备组织胞浆提取液，乙醚萃取去除提取液中所有的内源性类固醇激素，加入3H-DHT测定结合容量，并以等量和10倍量的T、E2、Po与3H-DHT竞争DHT结合容量。结果 前列腺组织胞浆单纯DHT结合容量是(0.0143±0.0022)nmol/g湿组织，T、E2与DHT竞争结合蛋白的作用较强，降低DHT结合容量的作用比较明显，Po的结合作用较弱，对结合容量的影响较小。 结论 前列腺组织胞浆中的DHT结合蛋白组成复杂，性质各异，有很强的结合DHT能力，与T、E2、Po相互作用，共同影响前列腺组织中DHT的状态。 【关键词】 前列腺 雄激素 雌激素 孕激素 雄激素结合蛋白 【Abstract】 Objectives This experiment is to quantify the total DHT (Dihydrotestosterone)-binding ability of DHT-binding proteins in prostate by assaying the DHT-binding capacity, and to study the change of DHT-binding capacity causing by the combination of T（Testosterone）, E2(Estrodiol), Po(Progestosterone) and DHT-binding protein, and to analyze preliminarily the effects of sex hormone on DHT-binding protein of prostate.Method To obtain cytosolic fracions from 20 normal tissues taken from accident-death corpses without serious diseases, and then to remove all the endogenous hormone from the cytosolic fracions by ether stripping.To assay the content of the bound 3H –DHT. To add the same dose of T、E2、Po to compete against 3H –DHT for DHT protein, and then to add 10 times dose of T、E2、Po to compete with 3H –DHT for DHT protein. Results The alone DHT-binding capacity of the cytosolic fractions in prostate is (0.0143±0.0022)nmol/g wet tissue. T and E2 have more obvious effect on reducing the DHT-binding capacity of the cytosolic fraction while Po has less effect on reducing the DHT-binding capacity. Conclusions There are many kinds of DHT-binding proteins in the cytosolic fraction in prostate tissues and their mechanisms are different. The proteins have strong DHT-binding capacity, and they interact with T、E2、Po to affect the DHT state in the prostate tissues. 【Key words】 Prostate Androgen Estrogen Progestosterone Androgen-binding protein 前列腺组织中存在多种二氢睾酮（Dihydrotestosterone，DHT）结合蛋白［１］，这些蛋白的性质和生理作用尚不十分明确。作者自2001年2月至2007年5月实验设计测定DHT结合容量来描述这些结合蛋白总的DHT结合能力，并以睾酮(CT)、雌二醇(E2)、孕酮(PO)