antioxidative peptides derived from enzyme hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection抗氧化肽来源于骨酶水解胶原蛋白后微波辅助酸预处理和氮气保护.pdfVIP

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antioxidative peptides derived from enzyme hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection抗氧化肽来源于骨酶水解胶原蛋白后微波辅助酸预处理和氮气保护.pdf

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antioxidative peptides derived from enzyme hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection抗氧化肽来源于骨酶水解胶原蛋白后微波辅助酸预处理和氮气保护

Int. J. Mol. Sci. 2010, 11, 4297-4308; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection Yun-Jian Lin 1,2, Guo-Wei Le 1,2,*, Jie-Yun Wang 1, Ya-Xin Li 1, Yong-Hui Shi 1,2 and Jin Sun 1,2 1 School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; E-Mails: yunjianl@163.com (Y.-J.L.); wang_jieyun@ (J.-Y.W.); liyaxin1220@163.com (Y.-X.L.) 2 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; E-Mails: yhshi@ (Y.-H.S.); sunj@ (J.S.) * Author to whom correspondence should be addressed; E-Mail: lgw@; Tel.: +86-510 Fax: +86-510 Received: 18 August 2010; in revised form: 21 October 2010 / Accepted: 21 October 2010 / Published: 1 November 2010 Abstract: This study focused on the preparation method of antioxidant peptides by enzymatic hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection. Phosphoric acid showed the highest ability of hydrolysis among the four other acids tested (hydrochloric acid, sulfuric acid and/or citric acid). The highest degree of hydrolysis (DH) was 9.5% using 4 mol/L phosphoric acid with a ratio of 1:6 under