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analysis of gene regulation in rabbit corneal epithelial cells induced by ultraviolet radiation分析基因调控在紫外线辐射引起的兔角膜上皮细胞
Int. J. Environ. Res. Public Health 2005, 2(1), 51–57
International Journal of
Environmental Research and Public Health
ISSN 1660-4601
© 2005 by MDPI
Analysis of Gene Regulation in Rabbit Corneal Epithelial Cells Induced
by Ultraviolet Radiation
Jacqueline J. Stevens1* 1 2 1 3
, Christian Rogers , Carolyn B. Howard , Caronda Moore , and Lai-Man Chan
1Molecular Biology Research Laboratory, 2Breast Cancer Research Laboratory, 3Chemistry Research Laboratory, NIH-
Center for Environmental Health, College of Science, Engineering and Technology, Jackson State University, 1400 J R
Lynch Street, Jackson, Mississippi 39217, USA.
*Correspondence to Dr. Jacqueline J. Stevens. Email: jacqueline.j.stevens@
Received: 15 November 2004 / Accepted: 06 February 2005 / Published: 30 April 2005
Abstract: Ultraviolet (UV)-induced cataracts are becoming a major environmental health concern because of the
possible decrease in the stratospheric ozone layer. Experiments were designed to isolate gene(s) affected by UV
irradiation in rabbit cornea tissues using fluorescent differential display-reverse transcription-polymerase chain
reaction (FDDRT-PCR). The epithelial cells were grown in standard medium for 2 or 4 hours post trea
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