anti-influenza activity of marchantins, macrocyclic bisbibenzyls contained in liverwortsmarchantins抗流感活动,大环的bisbibenzyls包含在苔类.pdfVIP

Anti-Influenza Activity of Marchantins, Macrocyclic Bisbibenzyls Contained in Liverworts 1 2 2 3 4 Yuma Iwai , Kouki Murakami , Yasuyuki Gomi , Toshihiro Hashimoto , Yoshinori Asakawa , Yoshinobu 2 2 1 1 1 Okuno , Toyokazu Ishikawa , Dai Hatakeyama , Noriko Echigo , Takashi Kuzuhara * 1 Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, Japan, 2 Research group, Research and Production Technology Division, Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University, Kanonji, Kagawa, Japan, 3 Laboratory of Pharmacognosy, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, Japan, 4 Laboratory of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, Japan Abstract The H1N1 influenza A virus of swine-origin caused pandemics throughout the world in 2009 and the highly pathogenic H5N1 avian influenza virus has also caused epidemics in Southeast Asia in recent years. The threat of influenza A thus remains a serious global health issue and novel drugs that target these viruses are highly desirable. Influenza A possesses an endonuclease within its RNA polymerase which comprises PA, PB1 and PB2 subunits. To identify potential new anti- influenza compounds in our current study, we screened 33 different types of phytochemicals using a PA endonuclease inhibition assay in vitro and an anti-influenza A virus assay. The marchantins are macrocyclic bisbibenzyls found in