antimicrobial peptide trichokonin vi-induced alterations in the morphological and nanomechanical properties of bacillus subtilis抗菌肽trichokonin vi-induced改变枯草芽孢杆菌的形态和纳米机械属性.pdfVIP

Antimicrobial Peptide Trichokonin VI-Induced Alterations in the Morphological and Nanomechanical Properties of Bacillus subtilis . . Hai-Nan Su , Zhi-Hua Chen , Xiao-Yan Song*, Xiu-Lan Chen, Mei Shi, Bai-Cheng Zhou, Xian Zhao, Yu- Zhong Zhang* The State Key Laboratory of Crystal Materials and the State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan, People’s Republic of China Abstract Antimicrobial peptides are promising alternative antimicrobial agents compared to conventional antibiotics. Understanding the mode of action is important for their further application. We examined the interaction between trichokonin VI, a peptaibol isolated from Trichoderma pseudokoningii, and Bacillus subtilis, a representative Gram-positive bacterium. Trichokonin VI was effective against B. subtilis with a minimal inhibitory concentration of 25 mM. Trichokonin VI exhibited a concentration- and time-dependent effect against B. subtilis, which was studied using atomic force microscopy. The cell wall of B. subtilis collapsed and the roughness increased upon treatment with trichokonin VI. Nanoindentation experiments revealed a progressive decrease in the stiffness of the cells. Furthermore, the membrane permeabilization effect of trichokonin VI on B. subtilis was monitored, and the results suggest that the leakage of intracellular materials is a possible mechanism of action for trichokonin VI, which led to alterations in the morphological and nanomechanical properties of B. subtilis. Citation: Su H-N, Chen Z-H, Song X-Y, Chen X-L, Shi M, et al. (2012) Antimicrobial Peptide Trichokonin VI-Induced Alterations in the Morphological and Nanomechanical Properties of Bacillu