an extensive circuitry for cell wall regulation in candida albicans一个广泛的电路在白色念珠菌细胞壁的监管.pdfVIP

An Extensive Circuitry for Cell Wall Regulation in Candida albicans 1,2 1,3 2 1,2 Jill R. Blankenship , Saranna Fanning , Jessica J. Hamaker , Aaron P. Mitchell * 1 Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America, 2 Department of Microbiology and Institute of Cancer Research, Columbia University, New York, New York, United States of America, 3 Department of Microbiology, University College Cork, Cork, Ireland Abstract Protein kinases play key roles in signaling and response to changes in the external environment. The ability of Candida albicans to quickly sense and respond to changes in its environment is key to its survival in the human host. Our guiding hypothesis was that creating and screening a set of protein kinase mutant strains would reveal signaling pathways that mediate stress response in C. albicans. A library of protein kinase mutant strains was created and screened for sensitivity to a variety of stresses. For the majority of stresses tested, stress response was largely conserved between C. albicans, Saccharomyces cerevisiae, and Schizosaccharomyces pombe. However, we identified eight protein kinases whose roles in cell wall regulation (CWR) were not expected from functions of their orthologs in the model fungi Saccharomyces cerevisiae and Schizosaccharomyces pombe. Analysis of the conserved roles of these protein kinases indicates that establishment of cell polarity is critical for CWR. In addition, we found that septins, crucial to budding, are both important for surviving and are mislocalized by cell wall stress. Our study shows an expanded role for protein kinase signaling in