有哪些信誉好的足球投注网站allele specific locked nucleic acid quantitative pcr (aslnaqpcr) an accurate and cost-effective assay to diagnose and quantify kras and braf mutation等位基因特定的锁核酸定量pcr(aslnaqpcr)一个精确的和具有成本效益的分析诊断和量化喀斯特braf突变.pdfVIP
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allele specific locked nucleic acid quantitative pcr (aslnaqpcr) an accurate and cost-effective assay to diagnose and quantify kras and braf mutation等位基因特定的锁核酸定量pcr(aslnaqpcr)一个精确的和具有成本效益的分析诊断和量化喀斯特braf突变
Allele Specific Locked Nucleic Acid Quantitative PCR
(ASLNAqPCR): An Accurate and Cost-Effective Assay to
Diagnose and Quantify KRAS and BRAF Mutation
1 1,2 1,2 1,2 3
Luca Morandi *, Dario de Biase , Michela Visani , Valentina Cesari , Giovanna De Maglio ,
3 2 1
Stefano Pizzolitto , Annalisa Pession , Giovanni Tallini *
`
1 Dipartimento di Ematologia e Scienze Oncologiche ‘‘L e A Seragnoli’’, Sezione di Anatomia Istologia e Citologia Patologica M. Malpighi Universita di Bologna-AUSL
`
Ospedale Bellaria, Bologna, Italy, 2 Dipartimento di Patologia Sperimentale Universita di Bologna, Bologna, Italy, 3 SOC Anatomia Patologica Azienda Ospedaliero-
Universitaria Santa Maria della Misericordia, Udine, Italy
Abstract
The use of tyrosine kinase inhibitors (TKIs) requires the testing for hot spot mutations of the molecular effectors
downstream the membrane-bound tyrosine kinases since their wild type status is expected for response to TKI therapy. We
report a novel assay that we have called Allele Specific Locked Nucleic Acid quantitative PCR (ASLNAqPCR). The assay uses
LNA-modified allele specific primers and LNA-modified beacon probes to increase sensitivity, specificity and to accurately
quantify mutations. We designed primers specific for codon 12/13 KRAS mutations and BRAF V600E, and validated the assay
with 300 routine samples from a variety of source
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