an engineered cardiac reporter cell line identifies human embryonic stem cell-derived myocardial precursors一个工程心脏记者细胞系识别人类胚胎干细胞心肌前体细胞.pdfVIP

An Engineered Cardiac Reporter Cell Line Identifies Human Embryonic Stem Cell-Derived Myocardial Precursors 1. 1. 1. 1 1 Carissa Ritner , Sharon S. Y. Wong , Frank W. King , Shirley S. Mihardja , Walter Liszewski , David J. Erle1,2, Randall J. Lee1,2,3, Harold S. Bernstein1,2,3,4* 1 Cardiovascular Research Institute, University of California San Francisco, San Francisco, California, United States of America, 2 Department of Medicine, University of California San Francisco, San Francisco, California, United States of America, 3 Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California, United States of America, 4 Department of Pediatrics, University of California San Francisco, San Francisco, California, United States of America Abstract Unlike some organs, the heart is unable to repair itself after injury. Human embryonic stem cells (hESCs) grow and divide indefinitely while maintaining the potential to develop into many tissues of the body. As such, they provide an unprecedented opportunity to treat human diseases characterized by tissue loss. We have identified early myocardial precursors derived from hESCs (hMPs) using an a-myosin heavy chain (aMHC)-GFP reporter line. We have demonstrated by immunocytochemistry and quantitative real-time PCR (qPCR) that reporter activation is restricted to hESC-derived cardiomyocytes (CMs) differentiated in vitro, and that hMPs give rise exclusively to muscle in an in vivo teratoma formation assay. We also demonstrate that the reporter does not interfere with hESC genomic stability. Importantly, we show that hMPs give rise