acute response of peripheral blood cell to autologous hematopoietic stem cell transplantation in type 1 diabetic patient急性反应外周血细胞的自体造血干细胞移植在1型糖尿病患者.pdfVIP

Acute Response of Peripheral Blood Cell to Autologous Hematopoietic Stem Cell Transplantation in Type 1 Diabetic Patient 1. 1. 3 3 1 1 1 Xiaofang Zhang , Lei Ye , Jiong Hu , Wei Tang , Ruixin Liu , Minglan Yang , Jie Hong , Weiqing 1 1,2 1 Wang , Guang Ning , Weiqiong Gu * 1 Shanghai Key Laboratory for Endocrine Tumors, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai E-institute for Endocrinology, School of Medicine, Shanghai Jiaotong University, Ruijin Hospital, Shanghai, People’s Republic of China, 2 Laboratory for Endocrine and Metabolic Diseases, Institute of Health Science, Shanghai Institutes for Biological Sciences, School of Medicine, Shanghai JiaoTong University, Chinese Academy of Sciences, Shanghai, People’s Republic of China, 3 Department of Hematology, School of Medicine, Shanghai Jiaotong University, Ruijin Hospital, Shanghai, People’s Republic of China Abstract Objective: Autologous nonmyeloablative hematopoietic stem cell transplantation (AHST) was the first therapeutic approach that can improve b cell function in type 1 diabetic (T1D) patients. This study was designed to investigate the potential mechanisms involved. Design and methods: We applied AHST to nine T1D patients diagnosed within six months and analyzed the acute responses in peripheral blood for lymphocyte subpopulation as well as for genomic expression profiling at the six-month follow-up. Results: We found six patients obtained insulin free (IF group) and three remained insulin dependent (ID group); C-peptide production was significantly higher in IF group compared to ID group