有哪些信誉好的足球投注网站a study on the analytical sensitivity of 6 bse tests used by the canadian bse reference laboratory研究6疯牛病的分析灵敏度测试使用的加拿大疯牛病参考实验室.pdfVIP
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a study on the analytical sensitivity of 6 bse tests used by the canadian bse reference laboratory研究6疯牛病的分析灵敏度测试使用的加拿大疯牛病参考实验室
A Study on the Analytical Sensitivity of 6 BSE Tests Used
by the Canadian BSE Reference Laboratory
John G. Gray, Sandor Dudas, Stefanie Czub*
Lethbridge Laboratory, Canadian Food Inspection Agency, Lethbridge, Alberta, Canada
Abstract
Bovine spongiform encephalopathy (BSE) surveillance programs have been employed in numerous countries to monitor BSE
prevalence and to protect animal and human health. Since 1999, the European Commission (EC) authorized the evaluation
and approval of 20 molecular based tests for the rapid detection of the pathological prion protein (PrPsc) in BSE infection.
The diagnostic sensitivity, convenience, and speed of these tests have made molecular diagnostics the preferred method for
BSE surveillance. The aim of this study was to determine the analytical sensitivity of 4 commercially available BSE rapid-test
kits, including the PrionicsH-Check WESTERN, the PrionicsH Check-PrioSTRIPTM, the BioRadH TeSeETM ELISA, and the IDEXXH
HerdChekTM EIA. Performances of these tests were then compared to 2 confirmatory tests, including the BioRadH TeSeETM
Western Blot and the modified Scrapie Associated Fibrils (SAF)/OIE Immunoblot . One 50% w/v homogenate was made from
experimentally generated C-type BSE brain tissues in ddH2O. Homogenates were diluted through a background of BSE-
negative brainstem homogenate. Masses of both positive and negative tissues in each dilution were calculated to maintain
the appropriate tissue amounts for each test platform. Specific concentrated homogenization buffer was added accordingly
to maintain the correct buffer condition for each test. ELISA-based tests were evaluated using their respective software/
detection platforms. Blot-protocols were evaluated by manual measurements of blot signal density. Detection limitations
were determined by fitted curves intersecting
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