a short–time scale colloidal system reveals early bacterial adhesion dynamics短期范围内胶体系统揭示了早期细菌粘附动力学.pdfVIP

PLoS BIOLOGY A Short–Time Scale Colloidal System Reveals Early Bacterial Adhesion Dynamics 1 2 2 1 2* Christophe Beloin , Ali Houry , Manuel Froment , Jean-Marc Ghigo , Nelly Henry ´ ´ ´ ´ 1 Unite de Genetique des Biofilms, Institut Pasteur, CNRS URA 2172, Paris, France, 2 Laboratoire Physico-Chimie Curie, Institut Curie, CNRS UMR 168, Universite Paris VI , Paris, France The development of bacteria on abiotic surfaces has important public health and sanitary consequences. However, despite several decades of study of bacterial adhesion to inert surfaces, the biophysical mechanisms governing this process remain poorly understood, due, in particular, to the lack of methodologies covering the appropriate time scale. Using micrometric colloidal surface particles and flow cytometry analysis, we developed a rapid multiparametric approach to studying early events in adhesion of the bacterium Escherichia coli. This approach simultaneously describes the kinetics and amplitude of early steps in adhesion, changes in physicochemical surface properties within the first few seconds of adhesion, and the self-association state of attached and free-floating cells. Examination of the role of three well-characterized E. coli surface adhesion factors upon attachment to colloidal surfaces—curli fimbriae, F- conjugative pilus, and Ag43 adhesin—showed clear-cut differences in the very initial phases of surface colonization for cell-bearing surface structures, all known to promote biofilm development. Our multiparametric analysis revealed