a limited number of antibody specificities mediate broad and potent serum neutralization in selected hiv-1 infected individuals有限数量的抗体特异性调节广泛而有效的血清中和在选定的hiv - 1感染者.pdfVIP

A Limited Number of Antibody Specificities Mediate Broad and Potent Serum Neutralization in Selected HIV-1 Infected Individuals 1 2 2 1 3 Laura M. Walker , Melissa D. Simek , Frances Priddy , Johannes S. Gach , Denise Wagner , Michael B. 1 3 1 1,4 Zwick , Sanjay K. Phogat , Pascal Poignard , Dennis R. Burton * 1 Department of Immunology and Microbial Science and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, California, United States of America, 2 International AIDS Vaccine Initiative, New York, New York, United States of America, 3 IAVI AIDS Vaccine Design and Development Laboratory, Brooklyn, New York, United States of America, 4 Ragon Institute of MGH, MIT, and Harvard, Boston, Massachusetts, United States of America Abstract A protective vaccine against HIV-1 will likely require the elicitation of a broadly neutralizing antibody (bNAb) response. Although the development of an immunogen that elicits such antibodies remains elusive, a proportion of HIV-1 infected individuals evolve broadly neutralizing serum responses over time, demonstrating that the human immune system can recognize and generate NAbs to conserved epitopes on the virus. Understanding the specificities that mediate broad neutralization will provide insight into which epitopes should be targeted for immunogen design and aid in the isolation of broadly neutralizing monoclonal antibodies from these donors. Here, we have used a number of new and established technologies to map the bNAb specificities in the sera of 19 donors who exhibit among the most potent cross-clade serum neut