细胞生物学实验方法习题（Cell biology, experimental methods, exercises）.docVIP

细胞生物学实验方法习题（Cell biology, experimental methods, exercises） Summary of basic questions and answers -- research methods of cell biology Noun interpretation section: 1 resolution: distinguish the minimum distance between two particles. 2, the body of the cell culture: the tissue removed after dispersion (mechanical method or enzymatic digestion) for individual cells in artificial culture conditions, the survival, growth, reproduction, subculture, growth, reproduction, and observe the process of contact inhibition and aging, the phenomenon of life. 3 、 cell line: in vitro culture, some cells have genetic mutation, and with cancer cell characteristics, loss of contact inhibition, it may pass indefinitely unlimited passage cells. 4, cell lines: in vitro generally can smoothly pass 40 - 50 generations, and still can maintain the original diploid number and contact inhibition behavior passage cells. 5, the primary cell culture: direct tissue from the organism through tissue grown monolayer cells, or by enzyme digestion or mechanical tissues were dispersed into single cells, were cultured in vitro in culture for the first time before passage is called primary culture. 6, cell culture: primary culture after the formation of the monolayer cultured cells were cultured to confluence (coming from a cell culture vessel with a certain ratio to some other cultured vessels transplantation of cells), or for the living space is insufficient or due to cell density caused by excessive nutrient depletion, will affect the cell growth, the isolation is called subcultured. 7 cell fusion: the fusion of two or more cells into a double core cell or multinucleated cell. Usually mediated by inactivated viruses or chemicals, or by electrical stimulation. 8 monoclonal antibodies: by cloning a single antibody secreting B lymphocyte, the antibody which is directed only to one antigen determinant has the characteristics of being specific and capable of mass production. Brief answer section: 1. What ar