琼脂糖凝胶电泳的制备及电泳分离核酸（Preparation of agarose gel electrophoresis and separation of nucleic acids by electrophoresis）.docVIP

下载本文档

8
0
约3.8千字
约 12页
2017-09-02 发布于河南
举报
版权申诉

琼脂糖凝胶电泳的制备及电泳分离核酸（Preparation of agarose gel electrophoresis and separation of nucleic acids by electrophoresis）.doc

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

琼脂糖凝胶电泳的制备及电泳分离核酸（Preparation of agarose gel electrophoresis and separation of nucleic acids by electrophoresis）

琼脂糖凝胶电泳的制备及电泳分离核酸（Preparation of agarose gel electrophoresis and separation of nucleic acids by electrophoresis） ======================================== page first ======================================== One . Experimental purpose : One Learn the operation of agarose gel electrophoresis. Two Grasp the principle of agarose gel electrophoresis ; Three Familiar with the use of laboratory instruments . Two . Experimental principle : Agarose is a naturally polymerized, long-chain molecule that dissolves in boiling water, Forty-five At the beginning of temperature, porous rigidity is formed The size of the gel pore depends on the concentration of agarose. DNA The molecules are negatively charged in the alkaline environment and swim toward the positive electrode under an applied electric field. DNA Molecules in Charge effect and molecular sieve effect in agarose gel electrophoresis. difference DNA Its molecular weight and When the configuration is different, the electrophoretic mobility is different, and different zones are distinguished. Agarose gel electrophoresis leave DNA The effect of molecular sieve effect on the migration rate is inversely proportional to the molecular weight. Ethidium bromide ( EB A flat molecule that emits fluorescence under ultraviolet radiation. EB With the DNA branch Daughter formation EB-DNA The fluorescence intensity of the composite is more free than that of the free state EB The fluorescence intensity of the emission is large Ten More than twice, and the fluorescence intensity and DNA Proportional to the content of. It can be detected by naked eye 5 ng with The DNA . Three. Experimental instruments and reagents: One Instruments: Micro pipettes, electrophoresis instruments, electrophoresis tanks, microwave ovens Two Reagent: Agarose: 1% ; Electrophoresis buffer Fifty * TAE Electrophoresis buffer Tris24.2g Glacial acetic acid 5.7ml , 0.25mol/L EDTA (pH8.0) 20ml Add distilled water to 100ml ) EB : 5 L / 100 ml TBE Three Electrophoresis mater