nanomechanical in situ monitoring of proteolysis of peptide by cathepsin b纳米机械原位监测蛋白质水解肽的组织蛋白酶b.pdfVIP

Nanomechanical In Situ Monitoring of Proteolysis of Peptide by Cathepsin B 1. 2. 3. 4 2 5 Taeyun Kwon *, Jinsung Park , Jaemoon Yang , Dae Sung Yoon , Sungsoo Na , Chang-Wan Kim , 3 3 6 2 Jin-Suck Suh , Yong-Min Huh , Seungjoo Haam *, Kilho Eom * 1 Research Institute for Engineering Technology, Korea University, Seoul, Republic of Korea, 2 Department of Mechanical Engineering, Korea University, Seoul, Republic of Korea, 3 Department of Radiology, College of Medicine, Yonsei University, Seoul, Republic of Korea, 4 Department of Biomedical Engineering, Yonsei University, Kangwon- do, Republic of Korea, 5 Department of Mechanical Engineering, Konkuk University, Seoul, Republic of Korea, 6 Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Republic of Korea Abstract Characterization and control of proteolysis of peptides by specific cellular protease is a priori requisite for effective drug discovery. Here, we report the nanomechanical, in situ monitoring of proteolysis of peptide chain attributed to protease (Cathepsin B) by using a resonant nanomechanical microcantilever immersed in a liquid. Specifically, the detection is based on measurement of resonant frequency shift arising from proteolysis of peptides (leading to decrease of cantilever’s overall mass, and consequently, increases in the resonance). It is shown that resonant microcantilever enables the quantification of proteolysis efficacy with respect to protease concentration. Remarkably, the nanomechanical, in situ monitoring of proteolysis allows us to gain insight into the kinetics of proteolysis of pep