nasa-approved rotary bioreactor enhances proliferation of human epidermal stem cells and supports formation of 3d epidermis-like structurenasa-approved旋转生物反应器提高人类表皮干细胞的增殖和支持3 d epidermis-like结构的形成.pdfVIP

NASA-Approved Rotary Bioreactor Enhances Proliferation of Human Epidermal Stem Cells and Supports Formation of 3D Epidermis-Like Structure 1. 1. 1 1,2 1,2 1 Xiao-hua Lei , Li-na Ning , Yu-jing Cao , Shuang Liu , Shou-bing Zhang , Zhi-fang Qiu , Hui-min 1,2 1,2 1,2 1 Hu , Hui-shan Zhang , Shu Liu , En-kui Duan * 1 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, 2 Graduate School, Chinese Academy of Sciences, Beijing, China Abstract The skin is susceptible to different injuries and diseases. One major obstacle in skin tissue engineering is how to develop functional three-dimensional (3D) substitute for damaged skin. Previous studies have proved a 3D dynamic simulated microgravity (SMG) culture system as a ‘‘stimulatory’’ environment for the proliferation and differentiation of stem cells. Here, we employed the NASA-approved rotary bioreactor to investigate the proliferation and differentiation of human epidermal stem cells (hEpSCs). hEpSCs were isolated from children foreskins and enriched by collecting epidermal stem cell colonies. Cytodex-3 micro-carriers and hEpSCs were co-cultured in the rotary bioreactor and 6-well dish for 15 days. The result showed that hEpSCs cultured in rotary bioreactor exhibited enhanced proliferation and viability surpassing those cultured in static conditions. Additionally, immunostaining analysis confirmed higher percentage of ki67 positive cells in rotary bioreactor compared with the static culture. In contrast, comparing with static culture, cells in the rotary bioreactor