multiple implants do not aggravate the tissue reaction in rat brain多个植入不加重老鼠大脑的组织反应.pdfVIP

Multiple Implants Do Not Aggravate the Tissue Reaction in Rat Brain ¨ Gustav Lind*, Lina Gallentoft, Nils Danielsen, Jens Schouenborg, Lina M. E. Pettersson Department of Experimental Medical Sciences, Neuronano Research Center, Medical Faculty, Lund University, Lund, Sweden Abstract Chronically implanted microelectrodes are an invaluable tool for neuroscientific research, allowing long term recordings in awake and behaving animals. It is known that all such electrodes will evoke a tissue reaction affected by its’ size, shape, surface structure, fixation mode and implantation method. However, the possible correlation between tissue reactions and the number of implanted electrodes is not clear. We implanted multiple wire bundles into the brain of rats and studied the correlation between the astrocytic and microglial reaction and the positioning of the electrode in relation to surrounding electrodes. We found that an electrode implanted in the middle of a row of implants is surrounded by a significantly smaller astrocytic scar than single ones. This possible interaction was only seen between implants within the same hemisphere, no interaction with the contralateral hemisphere was found. More importantly, we found no aggravation of tissue reactions as a result of a larger number of implants. These results highlight the possibility of implanting multiple electrodes without aggravating the glial scar surrounding each implant. ¨ Citation: Lind G, Gallentoft L, Danielsen N, Schouenborg J, Pettersson LME (2012) Multiple Implants Do Not Aggravate the Tissue Reaction in Rat Brain. PLoS ONE 7(10): e47509. doi:10.1371/journal.pone.0047509 Editor: Izumi Sugihara, Tokyo Medical and Dental University, Japan Received June 7, 2012; Accepted September 14, 2012; Published October 16, 2012 Copyright: 2012 Lind et al. This is an open-acce