molecular epidemiology of a pseudomonas aeruginosa hospital outbreak driven by a contaminated disinfectant-soap dispenser铜绿假单胞菌的分子流行病学医院疫情由被污染的消毒皂分配器.pdfVIP

Molecular Epidemiology of a Pseudomonas aeruginosa Hospital Outbreak Driven by a Contaminated Disinfectant-Soap Dispenser 1 1 1 1 2 1 Simone Lanini *, Silvia D’Arezzo , Vincenzo Puro , Lorena Martini , Francesco Imperi , Pierluca Piselli , 3 3 2 1 Marco Montanaro , Simonetta Paoletti , Paolo Visca , Giuseppe Ippolito 1 National Institute for Infectious Diseases ‘‘Lazzaro Spallanzani’’, I.R.C.C.S., Rome, Italy, 2 Department of Biology, University ‘‘Roma Tre’’, Rome, Italy, 3 UOC Ematologia ` Azienda Unita Sanitaria Locale di Viterbo, Viterbo, Italy Abstract Background and Objective: Pseudomonas aeruginosa infection represents a main cause of morbidity and mortality among immunocompromised patients. This study describes a fatal epidemic of P. aeruginosa that occurred in a hematology unit in Italy. Methods: Retrospective cohort study, prospective surveillance, auditing, extensive testing on healthcare workers and environmental investigation were performed to define the dynamics and potential causes of transmission. RAPD, macrorestriction analyses and sequence typing were used to define relationships between P. aeruginosa isolates. Results: Eighteen cases of infection were identified in the different phases of the investigation. Of these, five constitute a significant molecular cluster of infection. A P. aeruginosa strain with the same genetic fingerprint and sequence type (ST175) as clinical isolates strain was also isolated from a heavily contaminated triclosan soap dispenser. Discussion and Conclusions: Our results are consistent with the hypothesis that pati