molecular epidemiology of cross-species giardia duodenalis transmission in western uganda分子流行病学的跨物种鞭毛虫duodenalis传输在乌干达西部.pdfVIP

Molecular Epidemiology of Cross-Species Giardia duodenalis Transmission in Western Uganda 1 2 3 1 Amanda R. Johnston , Thomas R. Gillespie , Innocent B. Rwego , Traci L. Tranby McLachlan , Angela D. Kent4, Tony L. Goldberg3,5,6* 1 Center for Zoonoses and Infectious Disease Research and Department of Pathobiology, University of Illinois, Urbana, Illinois, United States of America, 2 Department of Environmental Studies and Global Health Institute, Emory University, Atlanta, Georgia, United States of America, 3 Department of Zoology, Makerere University, Kampala, Uganda, 4 Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana, Illinois, United States of America, 5 Department of Pathobiological Sciences, Nelson Institute for Environmental Studies, University of Wisconsin, Madison, Wisconsin, United States of America, 6 Center for Global Health, University of Wisconsin, Madison, Wisconsin, United States of America Abstract Background: Giardia duodenalis is prevalent in tropical settings where diverse opportunities exist for transmission between people and animals. We conducted a cross-sectional study of G. duodenalis in people, livestock, and wild primates near Kibale National Park, Uganda, where human-livestock-wildlife interaction is high due to habitat disturbance. Our goal was to infer the cross-species transmission potential of G. duodenalis using molecular methods and to investigate clinical consequences of infection. Methodology/Principal Findings: Real-time PCR on DNA extracted from fecal samples revealed a combined prevalence of G. duodenalis in people from three villages of 44/108 (40.7%), with prevalence reaching 67.5% in one village. Prevalence rates i