human papillomavirus genotype distribution in czech women and men with diseases etiologically linked to hpv人类乳头瘤病毒基因型分布在捷克女性和男性疾病的病因与人乳头状瘤病毒.pdfVIP

Human Papillomavirus Genotype Distribution in Czech Women and Men with Diseases Etiologically Linked to HPV 1 1 1 2 3 4 Ruth Tachezy *, Jana Smahelova , Martina Salakova , Marc Arbyn , Lukas Rob , Petr Skapa , Tomas Jirasek5, Eva Hamsikova1 1 Institute of Hematology and Blood Transfusion, Department of Experimental Virology, Prague, Czech Republic, 2 Unit of Cancer Epidemiology, Scientific Institute of Public Health, Brussels, Belgium, 3 Department of Obstetrics and Gynecology, Second Faculty of Medicine, Charles University, Prague, Czech Republic, 4 Department of Pathology and Molecular Medicine, Second Faculty of Medicine, Charles University, Prague, Czech Republic, 5 Department of Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic Abstract Background: The HPV prevalence and genotype distribution are important for the estimation of the impact of HPV-based cervical cancer screening and HPV vaccination on the incidence of diseases etiologically linked to HPVs. The HPV genotype distribution varies across different geographical regions. Therefore, we investigated the type-specific HPV prevalence in Czech women and men with anogenital diseases. Methods: We analyzed 157 squamous cell carcinoma samples, 695 precancerous lesion samples and 64 cervical, vulvar and anal condylomata acuminate samples. HPV detection and typing were performed by PCR with GP5+/6+ primers, reverse line blot assay and sequencing. Results: Thirty different HPV genotypes were detected in our study, HPV 16 being the most prevalent type both in precancerous lesions (45%) and squamous cell carcinomas (59%). In benign lesions, HPV 6 (72%) was the most common type. Altogether, 61% of carcinoma samples and 43% of precancerous lesion