high-throughput functional micrornas profiling by recombinant aav-based microrna sensor arrays高通量功能小分子核糖核酸分析通过重组aav-based微传感器阵列.pdfVIP

High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays 1,3. 2,6. 3,4 3 2 2 Wenhong Tian , Xiaoyan Dong , Xuerong Liu , Gang Wang , Zheyue Dong , Wei Shen , Gang 2 4 6 3 5 3 Zheng , Jianxin Lu , Jinzhong Chen , Yue Wang , Zhijian Wu *, Xiaobing Wu * 1 College of Life Science, Jilin University, Changchun, Jilin, China, 2 Beijing FivePlus Molecular Medicine Institute, Beijing, China, 3 State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China, 4 Wenzhou Medical College, Wenzhou, Zhejiang, China, 5 Unit on Ocular Gene Therapy, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States of America, 6 State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, China Abstract Background: microRNAs (miRNAs) are small and non-coding RNAs which play critical roles in physiological and pathological processes. A number of methods have been established to detect and quantify miRNA expression. However, method for high-throughput miRNA function detection is still lacking. Principal Findings: We describe an adeno-associated virus (AAV) vector-based microRNA (miRNA) sensor (Asensor) array for high-throughput functional miRNA profiling. Each Asensor contains a Gaussia luciferase (Gluc) and a firefly luciferase (Fluc) expression cassette to sense functional miRNA and to serve as an internal control respectively. Using this array, we acquired funct