high-throughput sequencing, characterization and detection of new and conserved cucumber mirnas高通量测序,表征和检测新的和守恒的黄瓜microrna.pdfVIP

High-Throughput Sequencing, Characterization and Detection of New and Conserved Cucumber miRNAs ´ ´ 1 1 2 ´ 1 ´ 1 German Martınez , Javier Forment , Cesar Llave , Vicente Pallas , Gustavo Gomez * ´ ´ ´ 1 Instituto de Biologıa Molecular y Celular de Plantas (IBMCP), Consejo Superior Investigaciones Cientıficas (CSIC) - Universidad Politecnica de Valencia (UPV), Valencia, ´ ´ Spain, 2 Centro de Investigaciones Biologicas (CIB), Consejo Superior Investigaciones Cientıficas (CSIC), Madrid, Spain Abstract Micro RNAS (miRNAs) are a class of endogenous small non coding RNAs involved in the post-transcriptional regulation of gene expression. In plants, a great number of conserved and specific miRNAs, mainly arising from model species, have been identified to date. However less is known about the diversity of these regulatory RNAs in vegetal species with agricultural and/or horticultural importance. Here we report a combined approach of bioinformatics prediction, high-throughput sequencing data and molecular methods to analyze miRNAs populations in cucumber (Cucumis sativus) plants. A set of 19 conserved and 6 known but non-conserved miRNA families were found in our cucumber small RNA dataset. We also identified 7 (3 with their miRNA* strand) not previously described miRNAs, candidates to be cucumber-specific. To validate their description these new C. sativus miRNAs were detected by northern blot hybridization. Additionally, potential targets for most conserved and new miRNAs were