有哪些信誉好的足球投注网站human papillomavirus type 18 e6 and e7 genes integrate into human hepatoma derived cell line hep g2人类乳头瘤病毒类型18 e6、e7基因融入人类肝癌细胞系消息灵通的g2派生而来.pdfVIP
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human papillomavirus type 18 e6 and e7 genes integrate into human hepatoma derived cell line hep g2人类乳头瘤病毒类型18 e6、e7基因融入人类肝癌细胞系消息灵通的g2派生而来
Human Papillomavirus Type 18 E6 and E7 Genes Integrate into Human Hepatoma Derived Cell Line Hep G2 1 1 2 1 1 1 1 1 Tianzhong Ma , Zhongjing Su , Ling Chen , Shuyan Liu , Ningxia Zhu , Lifeng Wen , Yan Yuan , Leili Lv , 1 3 1 Xiancai Chen , Jianmin Huang , Haibin Chen * 1 Department of Histology and Embryology, Shantou University Medical College, Guangdong Province, China, 2 Department of Biochemistry and Molecular Biology, Shantou University Medical College, Guangdong Province, China, 3 Pediatric Endocrine Unit, Massachusetts General Hospital for Children and Harvard Medical School, Boston, Massachusetts, United States of America Abstract Background and Objectives: Human papillomaviruses have been linked causally to some human cancers such as cervical carcinoma, but there is very little research addressing the effect of HPV infection on human liver cells. We chose the human hepatoma derived cell line Hep G2 to investigate whether HPV gene integration took place in liver cells as well. Methods: We applied PCR to detect the possible integration of HPV genes in Hep G2 cells. We also investigated the expression of the integrated E6 and E7 genes by using RT-PCR and Western blotting. Then, we silenced E6 and E7 expression and checked the cell proliferation and apoptosis in Hep G2 cells. Furthermore, we analyzed the potential genes involved in cell cycle and apoptosis regulatory pathways. Finally, we used in situ hybridization to detect HPV 16/18 in hepatocellular carcinoma samples. Results: Hep G2 cell line contains integrated HPV 18 DNA, leading to the expression of the E6 and E7 oncogenic proteins. Knockdow
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